{"id":6206,"date":"2026-05-06T09:56:27","date_gmt":"2026-05-06T09:56:27","guid":{"rendered":"https:\/\/agrosynapsis.com\/?p=6206"},"modified":"2026-05-06T09:56:31","modified_gmt":"2026-05-06T09:56:31","slug":"how-can-marker-assisted-breeding-remove-flanking-donor-dna-while-keeping-the-target-gene","status":"publish","type":"post","link":"https:\/\/agrosynapsis.com\/es\/how-can-marker-assisted-breeding-remove-flanking-donor-dna-while-keeping-the-target-gene\/","title":{"rendered":"How can marker-assisted breeding remove flanking donor DNA while keeping the target gene?"},"content":{"rendered":"<p>When introgressing, for example, resistance genes from wild species, unwanted donor segments can hitchhike along with your gene of interest\u2014a phenomenon known as linkage drag.<br><br>A structured marker-assisted breeding pipeline can help minimize this while retaining the resistance.<br><br>Here\u2019s how:<br><br>1. Foreground selection:<br>Start by selecting individuals that carry the target gene itself using a trait-linked marker in the first round of backcrossing (Bc1). This ensures all selected plants express the desired trait.<br><br>2. Recombinant selection:<br>Use markers flanking the target QTL on both sides to identify recombination events. For example, if markers are 5 cM from the QTL, roughly 5 recombinants are expected per 100 individuals at the first backcross (BC1).<br><br>Step 1: Screen BC1 progeny for the trait marker, then select recombinants on one side of the QTL.<br>Step 2: In BC2, repeat the process on the other side to recover recombinants from that side.<br><br>3. Result: By combining foreground and flanking-marker selection, you retain the target gene while minimizing linked donor DNA.<br><br>\ud83d\udca1Tip: The number of individuals to screen depends on the genetic distance of your flanking markers, and the optimal selection scheme should balance available genotyping resources and DNA extraction capacity.<br><br>Takeaway: Structured marker-assisted selection allows precise introgression of desired traits with minimal linkage drag, speeding up the development of improved varieties.<br><br>\ud83d\udc49 If you\u2019d like to be informed about the upcoming workshops organized by <a href=\"https:\/\/www.linkedin.com\/company\/agrosynapsis\/\">AgroSynapsis<\/a>, and receive early access and discounts, \ud835\uddf3\ud835\uddf6\ud835\uddf9\ud835\uddf9 \ud835\uddfc\ud835\ude02\ud835\ude01 \ud835\uddfc\ud835\ude02\ud835\uddff \ud835\ude00\ud835\uddf5\ud835\uddfc\ud835\uddff\ud835\ude01 \ud835\ude01\ud835\uddff\ud835\uddee\ud835\uddf6\ud835\uddfb\ud835\uddf6\ud835\uddfb\ud835\uddf4 \ud835\uddf6\ud835\uddfb\ud835\ude01\ud835\uddf2\ud835\uddff\ud835\uddf2\ud835\ude00\ud835\ude01 \ud835\uddf3\ud835\uddfc\ud835\uddff\ud835\uddfa here:<br><br><a href=\"https:\/\/lnkd.in\/g3tApqPz\">https:\/\/lnkd.in\/g3tApqPz<\/a><\/p>\n<div style=\"margin-top: 0px; margin-bottom: 0px;\" class=\"sharethis-inline-share-buttons\" ><\/div>","protected":false},"excerpt":{"rendered":"<p>Discover how marker-assisted selection can reduce linkage drag during introgression by combining foreground and flanking-marker strategies to retain only the desired gene<\/p>","protected":false},"author":2,"featured_media":6208,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[68],"tags":[],"class_list":["post-6206","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tips"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>How can marker-assisted breeding remove flanking donor DNA while keeping the target gene? 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