Applied Protocols
We help seed companies develop Applied Protocols for programs of Marker-Assisted Selection like:
- Gene Introgression
- Marker-assisted Backcross
- Early Selection in the nursery
- Gene Pyramiding
- Genomic Selection
- Pre-breeding
Discover our approach to streamlining and optimizing your molecular breeding logistics and applied protocols step by step
1. Define Objectives
We define clear breeding objectives and identify the specific traits you aim to introgress into the target variety.
2. Screen Genetic Resources
We assess available genetic resources, including germplasm collections, wild relatives, landraces, and other breeding materials, to identify potential donor species or varieties that carry the desired traits.
3. Select and Validate Markers
We choose appropriate molecular markers based on their polymorphism, abundance, and linkage to the target traits. We validate the selected molecular markers to ensure their association with the target traits across diverse genetic backgrounds.
4. Breeding Scheme
We develop a breeding scheme and selection strategy that integrates marker-assisted selection with phenotypic selection. We determine parameters like the optimal number of population size, selection pressure and number of generation etc.
5. Infrastructure and Resources
We indicate the necessary infrastructure, equipment, and resources for DNA extraction, sample collection, genotyping and data management.
6. Continuous Monitoring and Evaluation
We use a monitoring and evaluation system to track progress and adapt breeding strategies based on field trial results and feedback from breeders.
Heritability Estimation – we accurately measures how much of a trait’s variation is genetic, guiding breeders in focusing on traits with higher genetic gain potential.
